Author ' s response to reviews Title : Antioxidant genotoxic and antigenotoxic activity of

Title: Antioxidant genotoxic and antigenotoxic activity of Daphne gnidium leaf extracts Authors: Fadwa Chaabane FC ([email protected]) Jihed Boubaker JB ([email protected]) I confirm that this manuscript was submitted solely to this journal and is not published, in press, or submitted elsewhere. I have also prepared my paper and files in accordance with the journal's style and format requirements and all co-authors agree to its publication. We are willing this paper to be considered for publication in your journal as soon as possible. Methods As asked by reviewer 1:-We have listed the different concentrations used for each sample in each method in the corresponding section method.-We indicated the room temperature.-We indicated to how many hours this culture corresponded. Results-Plant materials were screened for the presence of tannins, flavonoids and coumarins by thin layer chromatography (TLC) on silica gel. Coumarins were detected under UV (366 nm) thanks to their blue fluorescence. However, we have used quantitative analysis only for polyphenols, flavonoides and tannins.-The sentence " According to …..as genotoxic if the IF exceeds 2 " was transferred to materials and methods section.-The sentence " Dose of 5μg/essay…..SOS response " was transferred to materials and methods section.-The sentence " the test implements two……spectrophotometrically at 560nm " was transferred to materials and methods section.-We changed 1 mg/ml by 0.5 mg/ml.-We added a figure (Fig.3) which illustrates the pro-oxidant effect of chloroform and petroleum ether extracts. Title-As asked by reviewer 2 we changed the title of the manuscript to " Antioxidant, genotoxic and antigenotoxic activitiy of Daphne gnidium leaf extracts " Background